Journal: Cancers
Article Title: Oral Bacteria, Virus and Fungi in Saliva and Tissue Samples from Adult Subjects with Oral Squamous Cell Carcinoma: An Umbrella Review
doi: 10.3390/cancers15235540
Figure Lengend Snippet: Data primarily concerning bacteria extracted and collected from the included studies that had carried out saliva testing in OSCC adult subjects. Studies: first author, year and journal of publication, reference, number and design of included studies, meta-analysis, assessed quality, funding (if any). Population characteristics: sample size, mean age, gender ratio, country of origin of the sample, risk factor and history of OPMD, history of malignancies, other comorbidities, and ongoing treatments. OSCC characteristics: macroscopic features, location, staging and grading, microscopic features, first diagnosis (primary site/metastatic lesion), time to onset, chemotherapy (yes/no), radiotherapy (yes/no). Intervention: number of sample(s), method(s) of sample collection, microorganisms identification technique, target. Outcome(s): type(s) of phylum, genus and species of bacterium detected, number or percentage of OSCC-positive cases.
Article Snippet: Mauceri, 2022 Cancers (Basel) Studies: n.11 CCS n.8 CS n.2 CSCS n.1 No meta-analysis Critically low quality No funding , Sample size: n.679 of case/n.480 of the healthy control group/n.153 of the OPMD control group/n.15 of the periodontitis control group Mean age: 56.8 y.o.; range 49.7–63.9 y.o. Gender ratio: 179M/113F/387MD Country: Taiwan n.445 China n.97 USA n.18 Sudan n.59 Japan n.60 Risk factors for OSCC: MD History of OPMD: MD Time to OPMD onset: MD Previous history of malignancies: MD Other comorbidities: MD Other ongoing treatments: MD , Macroscopic features: MD Location: MD Staging: MD Grading: MD Microscopic features: MD First diagnosis: MD Time to onset: MD Chemotherapy: MD Radiotherapy: MD , Sample(s): n.687 Method(s) of sample collection: Sputum n.448 Oral swab n.156 Oral rinse n.18 Saliva, subgingival plaque, tumor and healthy surface n.57 Microorganisms identification technique: QIAamp DNA Blood Mini kit n.347 QIAamp MinElute Virus Spin kit n.138 QIAamp Fast DNA Stool Mini kit n.10 FastDNA kit n.59 Gene Prep Star PI-80X device n.60 Modified QIAGEN DNA extraction method n.18 E.Z.N.A. soil DNA kit n.47 Target: Fungal ITS2 region n.59 V4 region n.280 V3-V4 region n.115 V3-V5 region n.138 V4-V5 region n.87 , ↑ Spirochaetes: ↑ Treponema ↑ Proteobacteria: ↑ Campylobacter ↓ Lautropia ↓ Haemophilus ↑ Eikenella ↑ Bacteroidetes: ↑ Alloprevotella ↑ Capnocytophaga ↑ PrevotellaTenericutes: ↑ Mycoplasma ↓ Firmicutes: MD n./% of OSCC case ↑ Centipeda ↑ Selenomonas ↑ Dialister ↑ Peptostreptococcus ↑ Filifactor ↑ Peptococcus ↑ Catonella ↑ Parvimonas ↓ Megasphaera ↓ Stomatobaculum ↓ Granulicatella ↓ Veillonella ↓ Streptococcus Type(s) of Streptococcus species detected: ↓ S. pneumoniae ↑ Fusobacteria: ↑ Fusobacterium Type(s) of Fusobacterium species detected: F. nucleatum ↓ Actinobacteria: MD n./% of OSCC case ↓ Scardovia ↓ Rothia ↓ Actinomyces.
Techniques: Bacteria, Control, Incubation, DNA Purification, Virus, Spectrophotometry, Gas Chromatography, Modification, DNA Extraction, Isolation
